Example 1:

IFN-gamma, human. Although the wells need not be as dark as this, this well represents a good assay. The spots are clearly defined but reflect their origin of diffusion, the rim of the well es clean and the background is even. There are no patterns to the spots.

Example 2:

IFN-gamma, human. The PVDF membrane has been rinsed with ethanol to improve coating with the capture antibody.

Example 3:

IFN-gamma, human. The PVDF membrane has been coated without pretreatment. Poorly defined spots and a tendency to give a messy rim.

Example 4:

B-cell EliSpot often gives huge, imprecisely outlined spots but this is a very nice IgG assay.

Example 5:

IFN-gamma, mouse. Poor coating and prolonged cell culture give patches of confluent spots, messy rim and overall questionable count results.