Typical images of wells

Product 90x90

Example 1:

IFN-gamma, human. Although the wells need not be as dark as this, this well represents a good assay. The spots are clearly defined but reflect their origin of diffusion, the rim of the well es clean and the background is even. There are no patterns to the spots.

Product 90x90

Example 2:

IFN-gamma, human. The PVDF membrane has been rinsed with ethanol to improve coating with the capture antibody.

Product 90x90

Example 3:

IFN-gamma, human. The PVDF membrane has been coated without pretreatment. Poorly defined spots and a tendency to give a messy rim.


Product 90x90

Example 4:

B-cell EliSpot often gives huge, imprecisely outlined spots but this is a very nice IgG assay.

Product 90x90

Example 5:

IFN-gamma, mouse. Poor coating and prolonged cell culture give patches of confluent spots, messy rim and overall questionable count results.