About EliSpot

 

 
 

EliSpot (Enzyme-Linked Immunosorbent Spot) is the most sensitive immunoassay and allows the monitoring of secreted cytokines even at the single T-cell level. With EliSpot it is possible to detect one specific cell out of 100.000 irrelevant cells, making this method much more sensitive than traditional ELISA applications.

 

 
 
  • Measurement of antigen-specific cytokine production of single T-cells
  • High sensitivity, high signal- to-noise ratio
  • Reproducible and reliable results within 24 hours
EliSpot  
   
EliSpot procedure

 

EliSpot  
 

Two-color diagnostic iSpot assays: Explore the future of Elispot diagnostics

EliSpot Assays

EliSpot Assays 
Red spot: cell secreting IL-2 only
Green spot: cell secreting γ-INF only (effector cell)
Double-stained spot: cell secreting both, γ-INF and IL-2 (memory cell)
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FAQ on iSpot and FluoroSpot

 
 
AID EliSpot Assay Kits

 

 
 

AID offers ready to use 96-well-plates, pre-coated with primary antibody, in a complete kit containing secondary antibody, conjugate and substrate. In addition, AID offers a selection of ready to use diagnostic kits, specific antigens already included.

 
 

Assays for routine diagnostics

Parameter Art.- No.

LymeSpot

ELSP 5510

Interferon-γ assay for the specific detection of T-cells secreted due to a Borrelia infection.

 

EBV-Spot

ELSP 5520

Interferon-γ assay for detection of Epstein-Barr-Virus (EBV) specific T-cells. Combination of EBV-lytic (BMLF1, BRLF1, BZLF1, ZEBRA) and EBV-latent (EBNA3a, LMP2) peptide mixes.

 

   
 


Basic research kits in 96-well format (Customized kits available!)

Parameter

Art.- No.

Human

 

Interferon-γ

ELSP 5000

TNF-α

ELSP 5080

Interleukin-2

ELSP 5050

Interleukin-4

ELSP 5060

Interleukin-5

ELSP 5010

Interleukin-6

ELSP 5020

Interleukin-10

ELSP 5040

Interleukin-12  

ELSP 5090

Interleukin-13

ELSP 5052

GM-CSF

ELSP 5053

Mouse

 

murine Interferon-γ

ELSP 5025

   
 
CE
   
 

For information about our other diagnostic products for example
Immobilized DNA probes, Immunoblots or Immunofluorescence,
please visit our website:

www.aid-diagnostika.com

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